mg63 human osteosarcoma cells  (Pasteur Institute)

Journal: RSC Advances

Article Title: Replicating the post-chemotherapy tumor microenvironment via biomimetic scaffolds to regulate MSC differentiation

doi: 10.1039/d6ra01031h

Figure Lengend Snippet: Biological characterizations of DOX-MSNCaP@PLGA-collagen and PLGA-collagen constructs. The accumulate release of DOX from DOX-MSNCaP@PLGA-collagen in PBS buffer with different pH (A). Cytotoxic assessment of MG63 cells after being cultured in scaffolds via live and dead staining. Cells were seed in the meshes and incubated in DMEM at pH 7.4 or pH 6.5 (B). Fluorescence microscopic observation on the intracellular distribution of DOX in MG63 cells after incubation with DOX-MSNCaP@PLGA-collagen scaffolds for 12 hours. Cell nucleus was represented blue fluorescence; DOX was represented red fluorescence; actin cytoskeleton was represented green fluorescence (C). DNA laddering analysis on the severity of DNA fragmentation in MG63 cells upon treatment of DOX-MSNCaP@PLGA-collagen for 12 hours (I: PLGA-collagen at pH 7.4, II: DOX-MSNCaP@PLGA-collagen at pH 7.4, III: DOX-MSNCaP@PLGA-collagen at pH 6.5) (D).

Article Snippet: Different types of cells were utilized in this work, including human bone marrow-derived mesenchymal stem cells (MSCs, passage 4) obtained from LONZA (MD, USA), and the human osteosarcoma cell line (MG63) purchased from Procell Life Science & Technology Co., Ltd (China; Catalog No. CL-0157).

Techniques: Construct, Cell Culture, Staining, Incubation, Fluorescence, DNA Laddering

Journal: RSC Advances

Article Title: Replicating the post-chemotherapy tumor microenvironment via biomimetic scaffolds to regulate MSC differentiation

doi: 10.1039/d6ra01031h

Figure Lengend Snippet: BrdU staining of MG63 upon treatment of DOX-MSNCaP@PLGA-collagen at pH 7.4 or pH 6.5. Scale bar: 50 µm.

Article Snippet: Different types of cells were utilized in this work, including human bone marrow-derived mesenchymal stem cells (MSCs, passage 4) obtained from LONZA (MD, USA), and the human osteosarcoma cell line (MG63) purchased from Procell Life Science & Technology Co., Ltd (China; Catalog No. CL-0157).

Techniques: BrdU Staining

Journal: Polymers

Article Title: Localized Combination Therapy Using Collagen–Hydroxyapatite Bone Grafts for Simultaneous Bone Cancer Inhibition and Tissue Regeneration

doi: 10.3390/polym17162239

Figure Lengend Snippet: Cytotoxicity effect on MG63 ( a ) and BMSCs ( b ) after 24 h of exposure to the tested samples’ extracts, evaluated via the release of LDH enzyme. Complete growth media was used as a negative control, and lysed cells were used as a positive control. Results are expressed as mean ± standard deviation (n ≥ 3, independent samples).

Article Snippet: For cell culture, human osteosarcoma cell line MG63 (ATCC CRL1427) was cultured in Dulbecco’s Modified Eagle’s Medium-DMEM supplemented with 1% non-essential amino acids, both from Sigma Aldrich, 10% fetal calf serum (FBS) from Thermo Fisher Scientific (Waltham, MA, USA), and 1% penicillin, streptomycin, and neomycin (all from Sigma Aldrich) at 37 °C with 5% CO 2 .

Techniques: Negative Control, Positive Control, Standard Deviation

Journal: Polymers

Article Title: Localized Combination Therapy Using Collagen–Hydroxyapatite Bone Grafts for Simultaneous Bone Cancer Inhibition and Tissue Regeneration

doi: 10.3390/polym17162239

Figure Lengend Snippet: Cellular viability of MG63 ( a ) and BMSCs ( b ) cultured with 100% extracts from each sample, for 1 day. Complete growth media was used as a control. The dashed line indicates the 70% cut-off for a toxic effect. Results are expressed as mean ± standard deviation (n ≥ 3, independent samples, * p < 0.05).

Article Snippet: For cell culture, human osteosarcoma cell line MG63 (ATCC CRL1427) was cultured in Dulbecco’s Modified Eagle’s Medium-DMEM supplemented with 1% non-essential amino acids, both from Sigma Aldrich, 10% fetal calf serum (FBS) from Thermo Fisher Scientific (Waltham, MA, USA), and 1% penicillin, streptomycin, and neomycin (all from Sigma Aldrich) at 37 °C with 5% CO 2 .

Techniques: Cell Culture, Control, Standard Deviation

Journal: Polymers

Article Title: Localized Combination Therapy Using Collagen–Hydroxyapatite Bone Grafts for Simultaneous Bone Cancer Inhibition and Tissue Regeneration

doi: 10.3390/polym17162239

Figure Lengend Snippet: Cellular viability of MG63 ( a ) and BMSCs ( b ) cultured with 100% extracts from each sample for 3 days. Results are expressed as mean ± standard deviation (n ≥ 3; independent samples; * p < 0.05).

Article Snippet: For cell culture, human osteosarcoma cell line MG63 (ATCC CRL1427) was cultured in Dulbecco’s Modified Eagle’s Medium-DMEM supplemented with 1% non-essential amino acids, both from Sigma Aldrich, 10% fetal calf serum (FBS) from Thermo Fisher Scientific (Waltham, MA, USA), and 1% penicillin, streptomycin, and neomycin (all from Sigma Aldrich) at 37 °C with 5% CO 2 .

Techniques: Cell Culture, Standard Deviation

Journal: Molecular Nutrition & Food Research

Article Title: Saucerneol Inhibits the Growth, Migration, and Invasion of Osteosarcoma Cells In Vitro and Prevents Metastasis‐Associated Osteolysis Ex Vivo

doi: 10.1002/mnfr.70187

Figure Lengend Snippet: Effects of Sauc on cell death, apoptotic cell morphology, and migration in human osteosarcoma cells. (A, B) MG63 cells (A) and SJSA‐1 cells (B) were seeded in 96‐well plates and cultured with Sauc at the indicated concentrations for 24 h. The numerical values of cytotoxicity are presented as a bar graph normalized to those of the control. (C, D) MG63 cells (C) and SJSA‐1 cells (D) were seeded in six‐well plates and cultured with Sauc at the indicated concentrations for 24 h. The morphological changes were observed using the Olympus CKX53 inverted microscope. Scale bar: 200 µm. (E, F) After 24 h of Sauc treatment of MG63 cells (E) and SJSA‐1 cells (F), cell migration was assessed using the wound healing assay. The migration rate is presented as a bar graph. Scale bar: 200 µm. All values are expressed as mean ± SD of the results from three independent experiments. * Indicates a statistically significant difference, with p < 0.05 compared to the control.

Article Snippet: Human osteosarcoma cell lines MG63 (#CRL‐1427) and SJSA‐1 (#CRL‐2098) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Migration, Cell Culture, Control, Inverted Microscopy, Wound Healing Assay